Optical fiber biosensor based on enzymatic coating matrix for catecholamines assessment in human urine
L. I. B. Silva, A. C. Freitas, T. A. P. Rocha-Santos, Piaget Institute (Portugal); M. E. Pereira, A. C. Duarte, University of Aveiro (Portugal)
An optical fiber (OF) biosensor has been developed and applied for simultaneous determination of catecholamines (dopamine, norepinephrine and epinephrine) in human urine. The developed analytical device shows a high potential for catecholamines quantification with a detection limit of 2.1, 2.6 and 3.4 pg mL-1 for dopamine, norepinephrine and epinephrine, respectively. The analytical performance of the OF biosensor was found to be similar to that of the High Performance Liquid Chromatography - Electrochemical Detector (HPLC-ED) regarding catecholamines determination in samples of human urine.
